Mammalian Expression

bacterial expression

yeast expression

Organelle Targeted FAPs

pcDNA3.1-COX4-COX8-dL5-2XG4S-mCer3
(Mitochondria Addgene 73208)

pcDNA3.1-kappa-myc-dL5-2XG4S-mCer-KDEL
(Endoplasmic Reticulum Addgene 73209)

pcDNA3.1-KozATG-dL5-2XG4S-mCer3
(Cytosolic Addgene 73207)

pcDNA3.1-myc-dL5-2XG4S-mCerSKL
(Peroxisomal Addgene 73210)

pcDNA3.1-NLS-myc-dL5-2XG4S-mCer3
(Nuclear Addgene 73205)

Actin-FAPs
pcDNA3.1-KozATG-dH6.2-2XG4S-actin
Addgene 73263

pcDNA3.1-KozATG-dL5-2XG4S-actin
Addgene 73262

Membrane Proteins
pcDNA3.1-kappa-dL5-2XG4S-TMst
(PDGFR TM anchored surface-expression FAP Addgene 73206)

pcDNA3.1-kappa-HA-dL5-BKa (KCNMA1)
(KCNMA1)

FAP dL5 Bacterial Expression
(Generally, we express from oxidizing strains such as Rosetta B-Gami2)
pET21a-10XHis-GST-HRV-dL5(YW)
(Addgene 74214)

Affibodies and Affi-FAP Constructs
(See Wang, Y et al, Bioconj. Chem. 2015)

pET21a-10XHis-GST-HRV-dHer1cysteineST
(double Her1-affibody Z1907 with cysteine at C-terminus Addgene 73218)

pET21a-10XHis-GST-HRV-dL5-Her1
(FAP-Affibody construct, Her1 Z1907 affibeody Addgene 73215)

pET21a-10XHis-GST-HRV-Her1-dL5-Her1
(Affibody-FAP-Affibody construct, Her1 affibeody Z1907 Addgene 73217)

pET21a-10XHis-GST-HRV-Her1-dL5
(Affibody-FAP construct, Her1 affibody Z1907 Addgene 73216)

 

Plasmid Maps


Transmembrane FAP
pcDNA3.1-kappa-myc-dL5-2XG4S-TMst
pcDNA3.1-kappa-dL5-2XG4S-TMst

Big Potassium Conductance Channel (BKa)
dL5-BKa pcDNA

Nuclear Localized FAP-mCer3 Fusion Protein
pcDNA3-NLS-myc-dL5-2XG4S-mCer

Mitochondrial Localized FAP-mCer3 Fusion Protein
pcDNA3.1-COX4-COX8-dL5-2XG4S-mCer

ER-Localized FAP-mCer3 Fusion Protein
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer-KDEL

Cytoplasmic Localized FAP-Mcer3 Fusion Protein –penetrates nucleus
pcDNA3.1-KozATG-dL5-2XG4S-mCer

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ApE Plasmid Editor
by M. Wayne Davis