Protocol list
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suspension and handling
How to properly suspend dyes and determine their concentration Protocol
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Surface Labeling FAP-Fusions
Surface selective labeling is achieved by adding a cell-excluded dye to living cells in a physiological buffer. Labeling is essentially instantaneous, if dye is added at mid-nanomolar concentrations. Protocol
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Intraceullar labeling fap-fusions
Total protein pool labeling is achieved by adding a cell-permeant dye to living cells in a physiological buffer. After equilibration for a short time, cell-permeant dye penetrates the cell to label intracellular expressed FAP sites. Labeling is rapid (10 min), and dye does not need to be washed out to achieve selective labeling.
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SUrface and Intracellular dye labeling combinations
Label distinctive pools of protein in different colors