suspension and handling
How to properly suspend dyes and determine their concentration Protocol
Surface Labeling FAP-Fusions
Surface selective labeling is achieved by adding a cell-excluded dye to living cells in a physiological buffer. Labeling is essentially instantaneous, if dye is added at mid-nanomolar concentrations. Protocol
Intraceullar labeling fap-fusions
Total protein pool labeling is achieved by adding a cell-permeant dye to living cells in a physiological buffer. After equilibration for a short time, cell-permeant dye penetrates the cell to label intracellular expressed FAP sites. Labeling is rapid (10 min), and dye does not need to be washed out to achieve selective labeling.
SUrface and Intracellular dye labeling combinations
Label distinctive pools of protein in different colors