Tools for Superresolution Imaging

Genetically targeted probes are very useful for imaging dynamic changes in living cells. Recently, it has been possible to image beyond the conventional diffraction limit of light by exploiting transitions between states in fluorescent molecules. Using fluorogen activating peptides, we have shown that STimulated Emission Depletion (STED) microscopy is practical in living cells, achieving a 2-3 fold enhancement over conventional microscopy. Using the equilibrium binding and activation of fluorogens by their cognate fluorogen activating peptides, we are developing new approaches for sparse labeling useful in single particle tracking and localization microscopy.

 

Confocal (Left) and STED (Right) images of actin filaments labeled with FAPs